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. 2013 Mar 26;8(3):e59693. doi: 10.1371/journal.pone.0059693

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© 2013 Chung et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The number of Annexin V positive cell didn’t increase in HK-2 cell cultured in hATMSCs- conditioned medium compared to HK-2 cell cultured in DMEM. Addition of CsA increased Annexin V positive cell both in DMED and hATMSCs-conditioned medium, and it was more frequently detected in HK-2 cell cultured in hATMSCs-conditioned medium compared to HK-2 cells cultured in DMEM. (B) The production of NO⁻ was increased in medium from hATMSCs treated with CsA compared with medium from hATMSCs cultured without CsA. (C) The 8-OHdG level was significantly increased in the medium from hATMSCs treated with CsA compared to medium from hATMSCs cultured withCsA-free media as well. hATMSCs; human adipose tissue derived mesenchymal stem cells.