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(A) RT-PCR analysis was performed for each sample in triplicate. Each patient sample (n = 10) was directly compared with all control samples (n = 11). (B) Enzyme-linked immunosorbent assay was used to measured intracellular IGFBP3 and IL6 and extracellular MMP3 level of production (NHM n = 8; VHM n = 8). Histogram reports the average ± SD after normalization for protein concentration. *p≤0.05.
