Figure 7. Analysis of resistance to blast fungus in OsPIANK1-overexpressing (OE) rice plants.

(A) Quantitative PCR analysis of OsPIANK1 expression in WT and OE plants (3#, 4#, and 6#). Data represent means ± SE of three independent experiments. (B) Lesions in leaves at 6 days after inoculation. The number of expanding lesions (Els) with an area greater than 0.5 mm² per leaf and their mean areas were determined using 10 leaves for WT (Nipponbare) and OsPIANK1-OE plants. Values represent means ± SE. (C) The amount of M. oryzae DNA in the WT and OsPIANK1-OE rice leaves. The leaves were harvested at 6 days after inoculation. Values represent the mean ± SE of three independent experiments. (D) Symptoms of rice blast in OsPIANK1-OE and WT rice plants grown in the greenhouse at 30 days after inoculation with spores of M. oryzae strain guy11. (**,*indicate significant differences between WT and OsPIANK1-OE plants at P<0.01 or P<0.05, respectively, as determined by the SNK test.).