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. 2013 Mar 26;8(3):e60609. doi: 10.1371/journal.pone.0060609

Figure 2. Quantification of bead aggregation.

Figure 2

(A) The protocol used for analyzing aggregation of beads. (B–D) Differential interference contrast (DIC) images of SVA beads coated with NT-biotin showing a typical field (B), the processed image after applying an intensity-threshold (C), and an enlarged image of the box (53.6×53.6 µm) shown in C (D) with single and double arrows indicating single and double beads. Scale bars represent 20 µm in all images. (E) Cartoon representation of the constructs used. Arrows indicate the PreScission protease cleavage site and b denotes the biotinylation sequence.