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. 2013 Mar 26;8(3):e60448. doi: 10.1371/journal.pone.0060448

Figure 4. Experimental evaluation of c*1043A>C mutation effect on miRNAs affinity.A.

Figure 4

: luciferase reporter constructs derived from pGL3-Control vector. Underlined and in bold is shown the location of the variant c.*1043A>C or altered nucleotide bases to destroy the seed overlapping region of miR-433 and miR-509-3p in the CFTR 3′UTR. B: luciferase expression from pLuc-CFTR-3′UTR or mutated form constructs transient transfected in different Panc-1 cells stably espressing the miR-433, miR-509-3p or the sh-3′UTR. Data reported here are the means ± St.Dev., **P<0.01