Figure 2. The interaction of initial RNA libraries and the Tat-peptide.

(A, B) Fluorescence intensities (FI) of the TMR-Tat at 590 nm mixed with G0 RNA libraries in a buffer containing 20 mM phosphate (pH 7.4), 100 mM NaCl, 1 mM MgCl₂, 20 ng/ µL tRNA, and 0.005% (v/v) Tween 20 at 37°C. TMR-Tat was mixed with varying concentrations of (A) th5N-G0 and (B) tc5N-G0 in the absence (blue) or presence of 3 mM theophylline (red) or 100 µM tetracycline (green).