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. 2013 Mar 26;8(3):e60413. doi: 10.1371/journal.pone.0060413

Figure 2. Mutations affect viral RNA synthesis at different temperatures.

Figure 2

Viral RNAs in MDCK cells infected with the different viruses (1×104 pfu ) were analyzed by real-time PCR. Cells were harvested at 16h p.i. (A) mRNA, cRNA, vRNA levels of PB1 from MDCK infected with A/PR8 & Pol1; (B) mRNA, cRNA, vRNA levels of PB2 from MDCK cells infected with A/PR8 & Pol1. P<0.05.