Figure 3. Mutations in PB1 and PB2 influenced polymerase activities of A/PR8 virus strain.

Mutant and wt polymerases activities at different temperatures were analyzed by minigenome assays. The luciferase expression was quantified as described in Materials and Methods. Mock-transfected cells were also analyzed as negative control. Cells were lysed after 48 h. The result was assayed by the luminometer. P<0.05