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. 2013 Mar 26;8(3):e60413. doi: 10.1371/journal.pone.0060413

Figure 5. Analysis of virus-specific CTL by tetramer-staining and Enzyme-linked immunospot (ELISPOT) for IFN-γ.

Figure 5

A, B. Tetramer-staining by CD8+−FITC and flu-tetramer-PE. The splenocytes were incubated with CD8+−FITC and flu-tetramer-PE. The stained cells were analyzed by flow cytometry with a Guava Easycyte. All FACS data were analyzed using FlowJo software v7.2.5. C,D. Elispot assay was done according to Mouse IFN- γ Elisport kit. Spots were counted by an Elispot reader (Cellular technology Ltd). P<0.05 1. Spleen cells (C57 immunized by A/PR8 intranasal, 1×104 pfu); 2. Spleen cells (C57 immunized by Pol2 intranasal, 1×104 pfu); 3.spleen cells (C57 immunized by Pol1 intranasal, 1×104 pfu); 4. Spleen cells (C57 immunized by inactivated A/PR8); 5. Spleen cells (C57 immunized by PBS)