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. 2013 Mar 26;8(3):e60528. doi: 10.1371/journal.pone.0060528

Figure 2. IRSp53 splicing does not affect podosome formation.

Figure 2

(A) Restoration of podosome formation by the expression of each IRSp53 splicing isoform. NIH-Src cells were transfected sequentially with the IRSp53 siRNA vector and the expression vectors, and were stained with rhodamine-phalloidin (red) to visualize actin filaments and with anti-IRSp53 (blue). Scale bar, 20 µm. (B) Percentage of cells with podosomes in (A). Data are means±SD from three independent experiments. Approximately 100 cells were counted in each determination. **P<0.01 (Student's t test). The C-terminal amino acid residues of the IRSp53 splicing variants are also indicated.