Table 3. List of top 5 genes identified by the MNI analysis at each time point in the gastrocnemius muscle of HFD-induced obese mice.

	Rank	Gene accession No.	Gene symbol	Description	Function	Fold change
Gastrocnemius muscle
2 week	1	NM_019574	Patz1	POZ (BTB) and AT hook containing zinc finger 1	Cancer	1.8
	2	NM_020610	Nrip3	Nuclear receptor interacting protein 3	Inflammation	0.42
	3	NM_024124	Hdac9	Histone deacetylase 9	Cancer	0.55
	4	XM_975536	Armc4	Armadillo repeat containing 4	Unknown	0.54
	5	NM_139226	Onecut3	One cut domain, family member 3	DNA binding	1.34
4 week	1	NM_153589	Tmem16b	Transmembrane protein 16B	Olfactory transduction	0.74
	2	NM_175540	Eda2r	Ectodysplasin A2 isoform receptor	Alopecia	0.88
	3	NM_008355	Il13	Interleukin 13	Inflammation	1.57
	4	NM_010608	Kcnk3	Potassium channel, subfamily K, member 3	Ion transport	1.22
	5	XM_129809	Ogfrl1	Opioid growth factor receptor-like 1	Unknown	0.7
8 week	1	NM_024124	Hdac9	Histone deacetylase 9	Cancer	0.6
	2	NM_011990	Slc7a11	Solute carrier family 7	Amino acid transport	0.7
	3	NM_175420	9330176C04Rik	RIKEN cDNA 9330176C04 gene	Unknown	2.17
	4	NM_016961	Mapk9	Mitogen activated protein kinase 9	Insulin resistance	0.59
	5	NM_027462	Wars2	Tryptophanyl tRNA synthetase 2 (mitochondrial)	Vasculogenesis	0.56
12 week	1	NM_008114	Gfi1	Growth factor independent 1B	Hematopoiesis	0.74
	2	NM_145435	Pyy	Peptide YY	Insulin resistance	1.49
	3	NM_138648	Olr1	Oxidized low density lipoprotein (lectin-like) receptor 1	Inflammation	0.33
	4	NM_177861	Tmem67	Transmembrane protein 67	Mental retardation	0.54
	5	XM_887155	Igsf10	Immunoglobulin superfamily, member 10	Unknown	0.42