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. 2013 Mar 26;8(3):e59591. doi: 10.1371/journal.pone.0059591

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© 2013 Lepiller et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Upper panel: Colony formation in soft agar seeded with PHH infected with HCMV-DB, but not with PHH uninfected or infected with heat-inactivated (HI) HCMV. Enhanced formation of colonies in soft agar that had been seeded with HepG2 cells infected with HCMV. None colony formation in soft agar that had been seeded with MRC-5 cells infected with HCMV, UV-inactivated HCMV or uninfected MRC-5 cells. Magnification: 100× (200× in upper left corner of picture). Lower panel: The histogram shows quantification of colony formation in soft agar as specified by the manufacturer (Cell Biolabs). Results represent means (± SD) of two independent experiments. ***P<0.001.