Figure 5. Deletion of hcpG and/or hcpC does not alter independent infection-induced signaling events.

CagA and activated MAPK (ERK2) expression in hcp mutants, WT G27MA and uninfected AGS cells over the course of infection. A shift in the fluorescence to the upper right quadrant in AGS cells infected with either WT or hcpC or hcpG mutants compared with uninfected AGS cells indicates increased CagA and activated-MAPK expression after infection. Results are representative of three experiments.