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. Author manuscript; available in PMC: 2013 Mar 26.
Published in final edited form as: J Immunol. 2010 Dec 22;186(3):1723–1734. doi: 10.4049/jimmunol.1002311

FIGURE 2.

FIGURE 2

Inhibition of miR-146a expression increases TNF-α response to TLR ligands. THP-1 cells transfected with 40 nM of miR-146a inhibitor for 24 h, along with mock-transfected controls, were washed with complete growth medium. Cells were then challenged with the various ligands for 5 h. A. Total RNA from cell pellets of mock- and miR-146a inhibitor-transfected cells were analyzed by qRT-PCR for miR-146a expression. B, Total RNAs were analyzed for TRAF6, IRAK-1, and unrelated lamin A/C (LMNA, negative control) mRNA expression in both mock- and miR-146a inhibitor-transfected cells by qRT-PCR. C, Western blot analysis for IRAK-1, TRAF6, and tubulin in the cell lysates collected 24 h after with or without (mock) miR-146a inhibitor transfection. D, TNF-α protein in supernatants of mock-transfected and miR-146a inhibitor-transfected cells and challenged with the various ligands shown at the foot of the graph were measured using ELISA. Data are expressed as mean ± SD of three independent experiments. *p < 0.05; **p < 0.01 compared with mock-transfected cells.