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. Author manuscript; available in PMC: 2013 Mar 26.
Published in final edited form as: J Immunol. 2010 Dec 22;186(3):1723–1734. doi: 10.4049/jimmunol.1002311

FIGURE 6.

FIGURE 6

IRAK-1 and TRAF6 knockdown results in decreased inflammatory response to microbial ligands. siRNA targeting IRAK-1 (A) or TRAF6 (B) were transfected into THP-1 cells, and knockdown efficiency was determined using qRT-PCR; lamin A/C (LMNA) was used as an unrelated mRNA control. Twenty-four hours after transfection, mock-transfected, IRAK-1–deficient, or TRAF6-deficient THP-1 cells were stimulated for 24 h with the indicated ligands shown at the foot of the graph (C–E). Cytokines released in the culture supernatants were determined by ELISA. Data are representative of three independent experiments and expressed as mean ± SD. *p < 0.05; **p < 0.01 compared with mock-transfected cell.