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. Author manuscript; available in PMC: 2013 Mar 26.
Published in final edited form as: J Immunol. 2010 Dec 22;186(3):1723–1734. doi: 10.4049/jimmunol.1002311

FIGURE 7.

FIGURE 7

Transfected miR-146a alone mimics LPS-induced cross-tolerance to various innate immune TLR ligands. THP-1 cells were transfected with 20 nM miR-146a mimic. They and mock-transfected cells were incubated for 24 h, washed with complete growth medium, and then challenged with indicated ligands for another 3 h. A, RNA isolates were prepared from cell pellets of mock and transfected cells followed by qRT-PCR for miR-146a expression normalized to RNU44. B, TNF-α protein measured using ELISA in supernatants from mock- and miR-146a-mimic-transfected cells. C, The RNA samples were also analyzed by qRT-PCR for mRNA expression of miR-146a targets TRAF6, IRAK-1, and unrelated lamin A/C (LMNA, negative control). Data are representative of three independent experiments and expressed as mean ± SD. *p < 0.05; **p < 0.01 compared with mock-transfected cells. Western blot analysis for IRAK-1, TRAF6, and tubulin in the cell lysates collected 24 h after with miR-146a-mimic transfection (D) or without (mock).