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. Author manuscript; available in PMC: 2013 Mar 26.
Published in final edited form as: J Immunol. 2010 Dec 22;186(3):1723–1734. doi: 10.4049/jimmunol.1002311

FIGURE 8.

FIGURE 8

Elevated levels of LPS-induced miR-146a contribute to resistance to heat-killed bacteria in the THP-1 cell model. A, THP-1 cells primed with 10 ng/ml LPS Se for 18 h (cross-tolerized, filled bars) and untreated controls incubated for the same time period (untolerized, open bars) were washed twice with PBS, then challenged with heat-killed E. coli or P. gingivalis at 1 × 106 CFU/ml for 3 h. B, THP-1 cells transfected with miR-146a mimic (40, 60, or 80 nM), followed by incubation for 24 h, and mock-transfected cells were washed with complete growth medium and challenged with heat-killed E. coli (1 × 106 CFU/ml). TNF-α protein in supernatants from mock-transfected and miR-146a mimic-transfected cells was measured using ELISA. Data are representative of three independent experiments and expressed as mean ± SD. *p < 0.05; **p < 0.01 compared with untolerized or mock-transfected cells.