Skip to main content
. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jan 4;1833(5):1096–1103. doi: 10.1016/j.bbamcr.2012.12.020

Figure 3.

Figure 3

Mig1-GFP is exported less efficiently in cells lacking Nup100 than control wild type cells. Mig1-GFP was expressed in wild type (-●-), msn5Δ (-○-), nup100Δ (-▼-), nup42Δ (-△-), and nup170Δ (-■-) cells in the presence of 2% dextrose. After confirming Mig1-GFP localization in the nucleus (t = 0 min), cells were pelleted, rinsed in media lacking a carbon source, resuspended in media containing 5% glycerol, and assayed for the percent of cells exhibiting nuclear fluorescence every 3–4 min. Error bars represent standard error of the mean.