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. Author manuscript; available in PMC: 2013 Mar 26.
Published in final edited form as: Med Oncol. 2009 Feb 24;27(1):91–97. doi: 10.1007/s12032-009-9178-z

Table 2.

Immunohistochemistry Techniques

Antibody Clone Company (location) Dilution (duration) Antigen retrieval method
EGFR H11 (monoclonal/mouse) Dako (Copenhagen, Denmark) 1:200 (30 min) Enzyme digestion (Proteinase K, Dako)
COX-2 COX229 (monoclonal/mouse) Zymed (San Francisco, CA) 1:200 (60 min) Heat, citrate buffer
p-EGFR 1H12 (monoclonal/mouse) Cell Signaling (Beverly, MA) 1:500 (overnight) Heat, citrate buffer
CD31 JC70A (monoclonal/mouse) Dako (Copenhagen, Denmark) 1:20 (60 min) Heat, citrate buffer
Ki-67 NCL Ki67 (monoclonal/mouse) Novocastra Lab (Newcastle, UK) 1:500 (overnight) Heat, citrate buffer