Figure 8. Priming with peptidoglycan results in neutrophil recruitment and proinflammatory cytokine production but does not protect mice against the lethal sequelae of PVM infection.
A. Percentage of GR1+ neutrophils detected in single cell suspensions from whole lung from mice inoculated with 100 µg PGN compared to 108 cfu L. reuteri (Lac). B. – F. Proinflammatory cytokines CXCL1, CCL3, CCL2, TNF-α, and CXCL10 detected in whole lung homogenates after first (day -14) and second (day - 7) intranasal inoculations with 100 µg PGN (filled symbols) or PBS/BSA control (open symbols); G. Survival of mice primed with PGN (100 µg / mouse; filled symbols) or PBS/BSA (PBS; open symbols) prior to inoculation with PVM on day 0; n = 10 mice/group; statistical significance, *p < 0.05.