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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Gastroenterology. 2012 Dec 22;144(4):789–798. doi: 10.1053/j.gastro.2012.12.025

Figure 3. Pharmacologic HO-1 induction prevents enteric microbiota induced colitis in Il10−/− mice.

Figure 3

(A) GF Il10−/− mice were injected IP with 5 mg/kg CoPP (n = 4) or DMSO (vehicle, n = 4) every 48 h for 7 days, then conventionalized for 14 days. (B) Western blot on colonic tissue from 2 GF mice after 7 days of CoPP administration. (C) At 14 days after CNV, colitis scores were determined in CoPP injected mice and compared with DMSO injected mice. Results are presented as the mean of four averaged scores from five regions of the large intestine. Bars represent mean ± SEM (4 mice/group). (D) Spontaneous IL-12 p40 secretion was determined by ELISA in 24 hour supernatants from colonic explants from CoPP and DMSO treated Il10−/− mice. Results represent mean ± SEM from three independent colonic explant cultures (n = 3 mice/group). *p < .05, versus DMSO (vehicle) injected mice.