Figure 3. HDM-sensitized Pglyrp1−/− mice have reduced cell infiltration and reduced goblet cell hyperplasia and metaplasia compared with WT mice.
(A) PAS staining of longitudinal lung sections along bronchial tree (Br) of untreated (top) and HDM-sensitized (bottom) WT (left) and Pglyrp1−/− (right) mice with massive inflammatory cell infiltrates (Ci) around bronchi, bronchioles, and pulmonary arteries (Ar) and veins (V) along the bronchial tree, with numerous large PAS+ mucin-producing goblet cells (G) lining the bronchial air spaces in WT, but not in Pglyrp1−/− mice; bar = 200 μm. (B) H&E staining of cellular infiltrates showing higher numbers of eosinophils (E) in WT than in Pglyrp1−/− mice; bar = 50 μm. (C) Numbers of PAS+ mucin-producing goblet cells lining the bronchial air spaces in WT and Pglyrp1−/− mice; means ± SEM of 6 mice/group; *, p < 0.00001; Pglyrp1−/− versus WT mice.