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Human T cells expressing Orai1-E106A remain localized to the HEV. A, Localization of human CD3+ T cells in the LN of a NOD.SCID mouse. This image shows CMTMR-labeled control (red) or eGFP-Orai1-E106A-transfected (green) human CD3+ T cells in a LN slice from a NOD.SCID mouse. HEVs were labeled with anti- peripheral node addressin (PNAd) antibody MECA-79. The inset shows labeled human cells localized in the HEV and cortex. B, The percentage of human T cells found within the HEV.
