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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Mar 1;190(7):3197–3206. doi: 10.4049/jimmunol.1202212

FIGURE 5.

FIGURE 5

Mouse T cells expressing Orai1-E106A cannot take up Ca2+ or home to the SLO of wildtype syngeneic mice. A, Fura-2 fluorescence ratios in transduced mouse CD3+ T cells loaded with fura-2. B, Homing of control mouse T cells following transduction. Successfully transduced cells (Thy1.1+) were purified via positive selection. Unbound mouse T cells (Thy1.1-) transduced with the CFP vector were labeled with CMTMR and Thy1.1- cells transduced with the eGFP-Orai1-E106A vector were labeled with CFSE and adoptively transferred. Whole organs were analyzed with T cell gates are drawn as indicated. C, Homing of positively isolated (Thy1.1+) mouse T cells. Cells were labeled: CFP+ with CMTMR and eGFP-Orai1-E106A+ with CFSE.