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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Mar 1;190(7):3197–3206. doi: 10.4049/jimmunol.1202212

FIGURE 7.

FIGURE 7

Orai1 function is required for transendothelial migration and LFA-1 activation. A, T cell migration measured in an uncoated transwell assay in response to different concentrations of recombinant mouse CCL21. Results are expressed as the percentage of cells of each subtype that migrated to the lower chamber of a transwell filter. In each panel, square symbols represent individual experiments showing control eGFP+ T cell migration and circles represent eGFP-Orai1-E106A+ T cells. n = 3 separate donors. B, T cell migration measured in EC-coated transwells in response to CCL21. n = 5. Statistics were determined with two-way ANCOVA. C, Representative flow cytometry histograms of active conformation LFA-1 following CCL21 treatment. Secondary control Ab represented in unfilled grey. D, Mean fluorescence intensities of human T cells from (C) stained with conformation-specific LFA-1 or VLA-4 antibodies. n = 3. E, Soluble ICAM-1/Fc binding to human T cells transfected with eGFP or eGFP-Orai1-E106A and treated with CCL21, normalized to ICAM-1/Fc binding to untreated controls. n =3.