Figure 5. Depletion of NKG2D+ NK cells rescues pregnancy and leads to loss of TNF-α production in WT and rIL-10 supplemented IL-10−/− mice in response to poly(I:C).
(A) Representative uterine horns from gd10 WT or rIL-10-supplemented IL-10−/− mice treated with anti-NKG2D antibody + saline or anti-NKG2D antibody + poly(I:C). Data represent mean ± S.E.M. (n= 4 mice/condition). NKG2D depletion abrogated fetal resorption as demonstrated by normal fetal units. (B) Assessment of NKG2D depletion and loss of TNF-α production was performed by gating on CD45+NK1.1+NKG2D+ uterine cells from saline or poly(I:C)-treated gd10 WT or rIL-10 + IL-10−/− mice injected i.p. with anti-NKG2D antibody. Dot plots represent data from 4 or more animals per condition.