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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Mol Microbiol. 2013 Mar 14;88(1):156–172. doi: 10.1111/mmi.12177

Figure 3. BvgS is the only kinase that phosphorylates BvgA in B. pertussis under typical growth conditions.

Figure 3

A. Schematic illustration of the B. pertussis bvgAS locus showing specific deletions of bvgA and bvgS used in this study. Also shown are the locations of the native BvgA-inducible and constitutive promoters and the Ptrc-v5 constitutive promoter added to the bvgA deletion strain QC3216 to drive bvgS expression.

B. Effect of specific chromosomal deletions on BvgA phosphorylation. B. pertussis strains BP536 (wild-type, lanes 1 and 2), QC2935 (ΔbvgS, lanes 3 and 4) and BP1526 (ΔbvgA lanes 5 and 6) grown in the absence (lanes 1, 3, and 5) and presence (lanes 2, 4, and 6) of MgSO4 were analyzed by Phos-tag™ gel-electrophoresis and Western blot. Control lanes contained purified wild-type BvgA incubated with (lane 7), or without (lane 8) Ac~P.