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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Mol Microbiol. 2013 Mar 14;88(1):156–172. doi: 10.1111/mmi.12177

Figure 5. Kinetics of in vivo phosphorylation of wild-type and mutant BvgA.

Figure 5

Liquid PLB cultures of B. pertussis strain QC3216 (ΔbvgA-Ptrc-v5-bvgS) harboring plasmids directing the synthesis of wild-type BvgA (pSS4983, lanes 1-6), or BvgAT194M (pBvgAT194M, lanes 7-12), were induced with 1 mM IPTG at t=0, and sampled at various times post induction. Cells were harvested by centrifugation, samples were prepared, and Phos-tag™ gel electrophoresis followed by Western blot analysis was performed as described above. Control lanes contained 1 pmol of purified BvgA (lane 13) or BvgA~P, (lane 14). The graph represents the ratio of BvgA~P relative to the total amount of BvgA protein for lanes 5, 6, 11 and 12.