. Author manuscript; available in PMC: 2014 Apr 1.

Published in final edited form as: Mol Microbiol. 2013 Mar 14;88(1):156–172. doi: 10.1111/mmi.12177

Table 1. Strains and plasmids used in this work.

Strain or plasmid	Relevant features	Source or reference
E. coli
DH5α	High-efficiency transformation	Bethesda Research Laboratories
BΛ21(ΔE3)[πΛψσE]	Expression strain for pET vectors, CamR	Studier et al., 1990
BΛ21(ΔE3)[πΛψσS]	Expression strain for pET vectors, CamR	Studier et al., 1990
SM10	Mobilization of RK2 oriT plasmids	Simon et al., 1983
B. pertussis
Tohama I	Patient isolate	Kasuga, et al. (1954)
BP536	Tohama I, Str^R, Nal^R	Stibitz and Yang (1991)
QC2935	BP536, ΔbvgS	This study
BP1526	BP536, ΔbvgA	Chen et al. (2010)
QC1416	BP536, pSS3967::P_fim3-i5c	Chen et al. (2010)
QC3216	BP536, ΔbvgA-P_trc-v5-bvgS	This study
Plasmids
pSS1827	Helper plasmid,for triparental mating, RP4 tra genes, Amp^R	Stibitz and Carbonetti (1994)
pQC1883	B. pertussis expression vector, Kan^R	This study
pSS4983	pQC1883::bvgA-wild-type, Kan^R	This study
pBvgA_D54N	pQC1883::bvgA_D54N, Kan^R	This study
pBvgA_T194M	pQC1883::bvgA_T194M, Kan^R	This study
pSS4661	Vector for allelic exchange in Bordetella, Kan^R	This study
pQC1805	pSS4661::ΔbvgS, kan^R	This study
pSS4871	pSS4661::ΔbvgA-P^c-bvgS, Kan^R	This study
pET21a(+)	Expression vector for BvgA in E. coli	EMD Millipore/Novagen
pET21a(+)-BvgA_wt	pET21a(+)::bvgA	This study
pET21a(+)-BvgA_D54N	pET21a(+)::bvgA_D54N	This study
pTE103	In vitro transcription vector	Elliot and Geiduscheck (1984)
pPfim3-15C	pTE103::Pfim3-15C	Chen et al. (2010)
pPfhaB	pTE103::PfhaB	Boucher et al. (1997)
pSS3967	luxCDABE promoter assay vector	Chen et al. (2010)
pQC1552	pSS3967::Pptx	This study
pQC1557	pSS3967::PfhaB	This study