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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Feb 27;190(7):3620–3628. doi: 10.4049/jimmunol.1102975

Figure 3. CaMKIα is sufficient to induce autophagy in macrophages.

Figure 3

RAW 264.7 cells were transfected with either a constitutively active CaMKIα(CaMKI293) or a kinase-deficient mutant (CaMKI293K49A) for the durations indicated. Total cellular lysate (20μg per lane) was isolated and analyzed for the induction of ATG7, LC3B, ATG5–12, CaMKIα, and actin by immunblot (representative blot of 3 independent experiments). Lipo=lipofectamine.