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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Feb 22;190(7):3590–3599. doi: 10.4049/jimmunol.1200860

FIGURE 3. Cyclic stretch stimulates IL-1β production via NLRP3-dependent signaling pathway.

FIGURE 3

A. Cyclic stretch (CS) induced NLRP3 inflammasome activation in AMs. AMs were exposed to 20% cyclic stretch for the indicated time. The assembly of NLRP3 inflammasome was detected using immunoprecipitation with anti-ASC Ab followed by immunoblotting for NLRP3, ASC, and caspase-1 cleavage product p10 fragments (Casp-1). The NLRP3 activation was confirmed by using immunoprecipitation with anti-NLRP3 Ab followed by immunoblotting for ASC, NLRP3, and Casp-1. B. Effects of depletion of NLRP3, IPAF, or AIM2 with respective siRNAs on IL-1β, IL-18 and mature Casp-1 production following 20% cyclic stretch for 4 h. IL-1β and IL-18 release in the culture supernatants (SN) of AMs and levels of Pro-IL-1β, Pro-IL-18, Pro-Casp-1 and Casp-1 in cell lysate (Lys) were determined by Western blot analysis. A scrambled siRNA (si Sc) was used as a negative control. C. The levels of IL-1β in cell-culture media were measured by ELISA. The graph shows the mean and SEM from 3 independent experiments. *p < 0.05 vs. the control (static) group. † p <0.05 vs. si Sc+CS group.