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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Feb 22;190(7):3590–3599. doi: 10.4049/jimmunol.1200860

FIGURE 4. Mitochondrial ROS are required for NLRP3 inflammasome activation following cyclic stretch.

FIGURE 4

A. Representative histograms of flow cytometry experiments demonstrating the effects of cyclic stretch and SS-31 on mitochondrial ROS generation. AMs were pretreated with SS-31 peptide (10 μM) for 24 h prior to stretch. AMs were stained with MitoSOX for 30 min and analyzed by flow cytometry. B. Quantitative data showing changes in mean fluorescent intensity (MFI) of MitoSOX following stretch and SS-31 treatment (n=3). C. SS-31 dose-dependently inhibited IL-1β release and caspase-1 activation following 20% cyclic stretch for 4 h. IL-1β release in the culture supernatants (SN) of AMs and levels of Pro-IL-1β, Pro-Casp-1 and Casp-1 in cell lysate (Lys) were determined by Western blot analysis. D. IL-1β in cell-culture media was measured by ELISA (n=3). E. Effects of rotenone on IL-1β release and caspase-1 activation following 20% cyclic stretch for 4 h. F. IL-1β in cell-culture media was measured by ELISA (n=3). *p < 0.05 vs. the control (static) group. † p <0.05 vs. CS alone group.