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. Author manuscript; available in PMC: 2014 Mar 5.
Published in final edited form as: Ann Neurol. 2013 Mar 5;73(2):266–280. doi: 10.1002/ana.23788

Figure 2.

Figure 2

Elevated expression of PH20, the soluble hyaluronidase in PH20, blocks OL maturation in vitro. OPCs were infected with lentiviruses carrying EGFP only (control; A, C), or viruses carrying EGFP plus Hyal1, Hyal2, Hyal5 (not shown) or PH20 (B, D). Cells were grown for 96 hours and stained for EGFP (green) and either HABP (A, B; red) or MBP (C, D; red). (E) Quantification of MBP expression by EGFP+ cells relative to cultures infected with the virus carrying EGFP alone. PH20 overexpression resulted in reduced HABP staining and inhibited OL maturation compared to cultures infected with only EGFP. HYAL2 overexpression partially inhibited OL maturation (78.48% of control). HYAL5 overexpression also partially inhibited OL maturation (70.08% of control). These experiments were performed 4 times with each data point assayed in triplicate wells. *p<0.02; **p<0.0009. Scale bar = 10 μm.