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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Mar 4;190(7):3417–3426. doi: 10.4049/jimmunol.1202563

Figure 4. PSMα3 inhibits clathrin-mediated endocytosis of DCs.

Figure 4

BM-DCs were incubated with 10 μM PSMα3 or with the indicated concentrations in the presence or absence of S. aureus cell lysate for 24 h and subsequently incubated with OVA-AlexaFluor647 (10 μg/ml) for 30 min (A), Lucifer yellow (2 mg/ml) for 10 min (B) or GFP-expressing Yersinia enterocolitica for 30 min (D) at 37 °C and analyzed by flow cytometry. C, Internalization of TLR4 was induced by LPS treatment (100 ng/ml) for 30 min in the presence or absence of PSMα3. TLR4 surface expression by BM-DCs was analyzed by flow cytometry. Representative histograms from three independent experiments are shown (A - C). Graphical summary of OVA- (A) or bacterial uptake (D); mean ± SD from three independent experiments. * indicates statistically significant differences compared to OVA-treated BM-DCs without PSMα3 (two-way ANOVA with Bonferroni post-test).