Figure 7. PSMα3 modulates cytokine production independently of mFPR2.
BM-DCs from wild type, FPR2−/−, and p110γ−/− mice were incubated with S. aureus cell lysate and 10 μM PSMα3 or MMK1 for 6 h (TNF) or 24 h (IL-12, IL-10) and cell culture supernatants were analyzed by ELISA (A) or were incubated with OVA-AlexaFluor647 for 30 min and OVA uptake was analyzed by flow cytometry (B). The data represent means of three independent experiments ± SD (A) or means of triplicates ± SD from one representative out of three independent experiments with similar results (B). * indicates statistically significant differences compared to S. aureus cell lysate treated BM-DCs or differences compared to the respective condition without inhibitor (one-way ANOVA with Bonferroni post-test).