Figure 1.

The Arabidopsis Mutant et2 Produces Diploid Male and Female Gametes.

(A) Volume-based pollen size distribution and microscopy analysis of wild-type (wt) and et2 mature pollen grains. Larger et2 pollen are indicated by arrows. Bar = 100 µm.

(B) DAPI staining of wild-type and et2 spores at the mature tricellular pollen stage and the uninuclear microspore stage. Bars = 10 µm.

(C) In vivo quantification of the gametophytic chromosome number in wild-type and et2 uninuclear microspores using ProWOX2-CENH3-GFP–mediated centromere labeling. Bar = 5 µm.

(D) ProWOX2-CENH3-GFP–mediated chromosome quantification in wild-type and et2 ovules at the binuclear megaspore stage. Bar = 5 µm.

[See online article for color version of this figure.]