Figure 6.

Cytological Analysis of frill1 Male Sporogenesis.

(A) Bright-field microscopy of mature pollen isolated from wild-type (wt) and frill1 flowers at anthesis and fluorescent visualization of sperm cells in mature pollen grains using the ProMGH3-H2B-GFP construct. Analysis of the male meiotic products in wild-type and et2 anthers through cytological tetrad stage meiocyte examination and mature pollen grain observations in the qrt1-1^−/− mutant background. Bars = 20 µm.

(B) DAPI-stained meiotic chromosome spreads of wild-type and frill1 male sporogenesis at different stages of the meiotic cell cycle. Bar = 10 µm.

(C) Chromosome spreading of somatic cells in premeiotic frill1 flower buds shows the ectopic formation of endomitotic polyploid cells. Bar = 5 µm.

[See online article for color version of this figure.]