Skip to main content
. 2013 Feb 26;25(2):421–437. doi: 10.1105/tpc.113.109223

Figure 1.

Figure 1.

Identification of the tie1-D Mutant.

(A) to (C) Top, from left to right, the 21-d-old wild-type plants and heterozygous and homozygous tie1-D mutants. The leaves of tie1-D were serrated and up-curled. Bars = 2 mm. Bottom, transverse sections of leaves from wild-type plants and heterozygous and homozygous tie1-D mutants. The leaves from homozygous tie1-D curled more than those of heterozygous tie1-D. Bars = 500 µm.

(D) and (E) Scanning electron micrographs of the leaf epidermal cells at the base of abaxial sides of mature leaves from the wild type (D) and tie1-D mutants (E). Bars = 50 µm.

(F) Distribution of cell size of the leaf epidermal cells from tie1-D mutants and wild-type control.

(G) Schematic representation of T-DNA insertion site in tie1-D mutant. The colored arrows represent genes and lines indicate the intergenic DNA region. The small red arrow in the intergenic region indicates the T-DNA insertion site in tie1-D. The four magenta arrowheads represent the four CaMV 35S enhancers of pSKI015 (Weigel et al., 2000). The small black arrows represent the DL1, P1, and P2 primers used in the cosegregation analysis. LB, T-DNA left border; RB, T-DNA right border; 4Enhancers, four CaMV 35S enhancers; bar, Basta resistance gene.

(H) Cosegregation analysis of T-DNA insertion with the hyponastic leaves. The 570-bp DNA bands were amplified from the wild-type genomic DNA and the 724-bp bands from tie1-D genomic DNA.

(I) The relative expression level of At4g28840 (TIE1) in the wild type and the homozygous tie1-D. The expression level of TIE1 in the wild type was set to 1.0. The error bars represent the sd of three biological replicates.

(J) Overexpression of At4g28840 by 35S promoter caused small and up-curled cotyledons. From left to right, two wild-type 7-d-old seedlings and four independent 35S-At4g28840 transgenic lines. Red arrow indicates a small and curly cotyledon. Bar = 1 mm.

(K) Recapitulation of tie1-D hyponastic leaf phenotype by overexpressing At4g28840 using a CaMV 35S promoter. Red arrow indicates a curly leaf. Bar = 1 mm.

(L) Recapitulation of tie1-D curly leaf phenotype by the overexpression of At4g28840 using the four CaMV 35S promoter enhancers and its own promoter. Red arrow indicates a curly leaf. Bar = 1 mm.