Figure 7.

Genetic Interaction between TIE1 and TCPs.

(A) to (F) Twenty-four-day-old wild-type plant (A), heterozygous tie1-D (B), homozygous tie1-D (C), homozygous jaw-5D (D), +/tie1-D jaw-5D double mutant (E), and tie1-D jaw-5D double mutant (F). The double mutants displayed deeply lobed leaves. Bars = 1 mm.

(G) to (L) The close-up views of the 6th leaves from (A) to (F). Bars = 1 mm.

(M) to (P) Ectopic shoots were frequently produced in the sinus of leaf serrations in the double mutants. Bars = 1 mm in (M) to (O) and 100 µm in (P).

(M) and (O) Close-up views of ectopic shoots.

(N) Two leaves with ectopic shoots from 30-d-old +/tie1-D jaw-5D double mutants.

(P) Scanning electron micrograph of ectopic shoot from 30-d-old +/tie1-D jaw-5D double mutant.

(Q) and (R) Eighteen-day-old plants of tcp3 tcp4 tcp10 triple mutant ([Q], top) and TIE1mEARVP16 transgenic line in the tcp3 tcp4 tcp10 background ([R], top) and the scanning electron micrograph of their leaf adaxial epidermal cells (bottom). Bars = 1 mm (top) and 50 µm (bottom).

(S) The 15-d-old seedlings of 35S-mTCP4-10, GFP-TIE1-15 35S-mTCP4-10, and GFP-TIE1-15 transgenic plants (from left to right). Bar = 1 mm.