Table 2. Molecular Interactions between Three California Poppy B, C, and/or E Proteins Tested by TriFC Using Two Tagged Proteins and a Third Untagged (Silent) Version.
| Combination | YFP N | YFP C | Silent Partner | Interaction |
|---|---|---|---|---|
| BBC | ||||
| SEI/DEF1/AG1 | AG1 | DEF1 | SEI | − |
| AG1 | DEF1 | sei-1 | − | |
| SEI/DEF2/AG1 | AG1 | DEF2 | SEI | +++a |
| AG1 | DEF2 | sei-1 | −a | |
| SEI/DEF3/AG1 | AG1 | DEF3 | SEI | +++ |
| AG1 | DEF3 | sei-1 | +++ | |
| AG1 | DEF3 | mSEI | +++ | |
| DEF3 | AG1 | SEI | − | |
| DEF3 | AG1 | sei-1 | − | |
| BBE | ||||
| SEI/DEF1/SEP3 | SEP3 | SEI | DEF1 | +++a |
| SEP3 | sei-1 | DEF1 | −a | |
| SEP3 | DEF1 | SEI | − | |
| SEP3 | DEF1 | sei-1 | − | |
| SEI | SEP3 | DEF1 | − | |
| sei-1 | SEP3 | DEF1 | − | |
| SEI/DEF2/SEP3 | SEP3 | SEI | DEF2 | +++ |
| SEP3 | sei-1 | DEF2 | +++ | |
| DEF2 | SEP3 | SEI | +++ | |
| DEF2 | SEP3 | sei-1 | +++ | |
| SEI/DEF3/SEP3 | SEP3 | DEF3 | SEI | +++ |
| SEP3 | DEF3 | sei-1 | +++ | |
| DEF3 | SEP3 | SEI | − | |
| DEF3 | SEP3 | sei-1 | − | |
| BCE | ||||
| SEI/AG1/SEP3 | AG1 | SEP3 | SEI | +++a |
| AG1 | SEP3 | sei-1 | −a | |
| AG1 | SEP3 | mSEI | −a |
Interactions that differ between SEI and sei-1.
Some of the interactions are illustrated in Figures 3B to 3I, 3M to 3O, and Supplemental Figure 9 online. +++, Strong interaction; −, no interaction.