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. 2013 Feb 15;25(2):454–469. doi: 10.1105/tpc.112.104760

Figure 1.

Figure 1.

Expression Analysis of VIN3 Gene Family Members during the Course of Vernalization.

(A) mRNA expression analysis of VIN3 gene family members during the course of vernalization using real-time RT-PCR. NV, nonvernalized; 10V, 10 d of vernalization; 20V, 20 d of vernalization; 30V, 30 d of vernalization; 40V, 40 d of vernalization; 40VT10, 40 d of vernalization followed by 10 d of normal growth temperature. Relative fold change was determined by normalization with the levels of PP2A gene. Real-time PCR experiments were performed with three biological replicates, and error bars indicate sd. Primer sequence information used for real-time RT-PCR analysis is shown in Supplemental Table 1 online.

(B) Immunoblot analysis of VIN3 family of proteins during the course of vernalization. NV, nonvernalized; 40V, 40 d of vernalization; 40VT10, 40 d of vernalization followed by 10 d of normal growth temperature. Corresponding insertional mutants were used as negative controls for VIN3, VIL1/VRN5, and VIL2/VEL1 immunoblots. Two independent RNAi lines (#5 and #9) were used as negative controls for VIL3/VEL2 immunoblots. WT, the wild type.