Figure 9.

Vernalization-Mediated Enrichments of PRC2 at Chromatins of FLC Gene Family Members.

ChIP assay using GFP-CLF transgenic plants to address the enrichment of CLF at chromatins of FLC gene family members during the course of vernalization. Nontransgenic plants (NT) were compared for the specificity of precipitation. Relative positions of primers used for each chromatin are depicted in Figure 5A. NV, nonvernalized; 20V, 20 d of vernalization; 40V, 40 d of vernalization; 40VT10, 40 d of vernalization followed by 10 d of normal growth temperature. Real-time PCR experiments were performed with three biological replicates and error bars indicate sd. AG was used as a control. First, relative enrichment of each fragment was calculated based on comparison to quantitative PCR using INPUT material. Second, relative enrichment was calculated by comparison to the AG proximal promoter control (Schubert et al., 2006). To show relative fold change, the value of nonvernalization time point in nontransgenic plants was converted to 1 and relative fold changes during the course of vernalization were presented.

[See online article for color version of this figure.]