Table 2. Progeny Analysis of Crosses Involving Three S2P:AmiS2-SLF1/S2S3 Transgenic Plants.
| Male Parent | Copy No. | Female Parent | Observed Ratioa | Progeny S-Genotype | Expected Ratioa | χ2 | P Value |
|---|---|---|---|---|---|---|---|
| S2P:AmiS2-SLF1/S2S3-4 | 1.29 ± 0.09 | S3S3 | 10:25 | S2S3 | 1:2 | 1.00 | 0.32 |
| S2P:AmiS2-SLF1/S2S3-4 | 1.29 ± 0.09 | S2S2 | 8:24 | S2S3 | 1:2 | 2.67 | 0.10 |
| S2P:AmiS2-SLF1/S2S3-4 | 1.29 ± 0.09 | AS-S3R/S3S3b | 10:24 | S2S3 | 1:2 | 0.67 | 0.41 |
| 2:8 | S3S3 | 1:2 | 2.00 | 0.16 | |||
| S2P:AmiS2-SLF1/S2S3-13 | 2.28 ± 0.38 | S3S3 | 14:29 | S2S3 | 1:2 | 0.03 | 0.85 |
| S2P:AmiS2-SLF1/S2S3-13 | 2.28 ± 0.38 | S2S2 | 14:31 | S2S3 | 1:2 | 0.29 | 0.59 |
| S2P:AmiS2-SLF1/S2S3-13 | 2.28 ± 0.38 | AS-S3R/S3S3b | 8:18 | S2S3 | 1:2 | 0.11 | 0.74 |
| 7:14 | S3S3 | 1:2 | 0.00 | 1.00 | |||
| S2P:AmiS2-SLF1/S2S3-13 | 2.28 ± 0.38 | S5S11 | 10:27 | S2S5 | 1:2 | 1.81 | 0.18 |
| 10:24 | S2S11 | 1:2 | 0.67 | 0.41 | |||
| 8:16 | S3S5 | 1:2 | 0.00 | 1.00 | |||
| 9:19 | S3S11 | 1:2 | 0.05 | 0.82 | |||
| S2P:AmiS2-SLF1/S2S3-13 | 2.28 ± 0.38 | S7S13 | 9:23 | S2S7 | 1:2 | 1.09 | 0.30 |
| 5:10 | S2S13 | 1:2 | 0.00 | 1.00 | |||
| 6:12 | S3S7 | 1:2 | 0.00 | 1.00 | |||
| 5:15 | S3S13 | 1:2 | 1.67 | 0.20 | |||
| S2P:AmiS2-SLF1/S2S3-24 | 0.93 ± 0.04 | S3S3 | 14:32 | S2S3 | 1:2 | 0.50 | 0.48 |
| S2P:AmiS2-SLF1/S2S3-24 | 0.93 ± 0.04 | S2S2 | 11:30 | S2S3 | 1:2 | 2.13 | 0.14 |
| S2P:AmiS2-SLF1/S2S3-24 | 0.93 ± 0.04 | AS-S3R/S3S3b | 4:11 | S2S3 | 1:2 | 0.82 | 0.37 |
| 9:21 | S3S3 | 1:2 | 0.43 | 0.51 | |||
| S2P:AmiS2-SLF1/S2S3-24 | 0.93 ± 0.04 | S5S11 | 5:14 | S2S5 | 1:2 | 1.14 | 0.29 |
| 3:8 | S2S11 | 1:2 | 0.50 | 0.48 | |||
| 6:11 | S3S5 | 1:2 | 0.09 | 0.76 | |||
| 8:17 | S3S11 | 1:2 | 0.06 | 0.81 | |||
| S2P:AmiS2-SLF1/S2S3-24 | 0.93 ± 0.04 | S7S13 | 3:7 | S2S7 | 1:2 | 0.14 | 0.71 |
| 2:8 | S2S13 | 1:2 | 2.00 | 0.16 | |||
| 8:17 | S3S7 | 1:2 | 0.06 | 0.81 | |||
| 9:15 | S3S13 | 1:2 | 0.60 | 0.44 |
Number of plants that had inherited AmiS2-SLF1 transgene/total number of plants examined.
AS-S3R/S3S3 stands for AS-S3-RNase/S3S3.