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. 2012 Feb 12;25(2):517–534. doi: 10.1105/tpc.112.105767

Figure 1.

Figure 1.

Phenotypic Characterization of FyPP1 and FyPP3 Loss- and Gain-of-Function Mutants in ABA Responses.

(A) Germination and growth of Col, f1, and f3 seeds and seeds from self-pollinated f1−/+f3 plants incubated on GM plates with 0 (top panels) or 1 μM ABA (bottom panels) for 5 d. Seeds from self-pollinated f1−/+f3 plants were more sensitive to ABA than Col seeds.

(B) Germination of Col, f1, and f3 seeds and seeds from self-pollinated f1−/+f3 plants incubated on GM plates with varying concentrations of ABA (0, 0.5, 1, 2.5, and 5 μM) for 5 d. Seeds from self-pollinated f1−/+f3 plants were more sensitive to ABA than Col. Germination percentages were determined from three independent experiments, with more than 100 seeds per line for each experiment. Values are means ± sd.

(C) Greening of Col, f1, and f3 seeds and seeds from self-pollinated f1−/+f3 plants incubated on GM plates with 0 or 1 μM ABA for 5 d. Seeds from self-pollinated f1−/+f3 plants were more sensitive to ABA than Col seeds. Greening was determined with an average of >100 seeds from three independent experiments. Values are means ± sd.

(D) Germination of Col, f1, and F3Ri/f1 seeds incubated on GM plates with varying concentrations of ABA (0, 0.5, 1, 2.5, and 5 μM) for 5 d. All GM plates contained 0.1% ethanol. Ethanol-induced F3Ri/f1 seeds were more sensitive to ABA than Col or f1 seeds. Germination percentage was determined from three independent experiments, with more than 150 seeds per line for each experiment. Values are means ± sd.

(E) Greening of Col, f1, and F3Ri/f1 seeds incubated on GM plates (plus 0.1% ethanol) with 0 or 1 μM ABA for 5 d. Ethanol-induced F3Ri/f1 seeds were more sensitive to ABA than Col seeds or f1 seeds. Greening was determined with an average of >150 seeds from three independent experiments. Values are means ± sd.

(F) Germination of Col, F1DN, F3DN, F1OE, and F3OE seeds incubated on GM plates with varying concentrations of ABA (0, 0.5, 1, 2.5, and 5 μM) for 5 d. F1DN and F3DN seeds were more sensitive to ABA, while F1OE and F3OE seeds were less sensitive to ABA than Col. Germination percentages were determined from three independent experiments, with more than 100 seeds per line for each experiment. Values are means ± sd.

(G) Greening of Col, F1OE, F3OE, F1DN, and F3DN seeds incubated on GM plates with 0 or 1 μM ABA for 5 d. F1DN and F3DN seeds were more sensitive to ABA, while F1OE and F3OE were less sensitive to ABA than Col seeds. Greening was determined with an average of >100 seeds from three independent experiments. Values are means ± sd. Asterisks indicate the levels of statistical significance as determined by Student’s t test: *P < 0.01 versus Col.