Figure 2.

Genetic Interaction between ABI5 and FyPPs.

(A) Germination and growth of seeds of Col, abi5, and self-pollinated f1^−/+ f3 and f1^−/+ f3 abi5 plants incubated on GM plates with 0 or 0.5 µM ABA for 5 d in white light. Seeds from self-pollinated f1^−/+f3 plants showed increased sensitivity to ABA, while abi5-1 seeds were insensitive to ABA compared with Col controls. Seeds from self-pollinated f1^−/+ f3 abi5-1 plants were insensitive to ABA, similar to abi5-1 seeds.

(B) Loss of FyPP1 and FyPP3 activity enhances the ABA-related phenotypes of ABI5OE seeds. Top panel shows seedlings grown on GM plates without ABA. The ungerminated seeds (25.2%) from self-pollinated f1^−/+ f3 ABI5OE plants were further phenotyped and genotyped and confirmed to be f1 f3 ABI5OE homozygotes. Bottom panel shows the seedlings grown on GM plates containing 0.5 µM ABA. The germination and greening percentages of each line are shown at the bottom of each panel, and the number of seeds used for the calculation is shown in parentheses.

(C) Growth of Col, F3OE, ABI5OE, and F3OE ABI5OE seeds on GM plates with 0 or 0.5 µM ABA. Overexpression of FyPP3 rescued the ABA hypersensitivity phenotype of ABI5OE seeds. The germination and greening percentages of each line are shown at the bottom of each panel, and the number of seeds used for the calculation is shown in parentheses.