Figure 10.

Schematic Representation of Phototropin-Dependent Cp-Actin Filament Dynamics.

(A) Cp-actin filaments in low light–adapted cells.

(B) and (C) Asymmetric cp-actin filaments during the avoidance response induced by microbeam irradiation with strong blue light at the left half of the chloroplast as shown in (A). Cp-actin filaments disappeared when they were irradiated with strong blue light and reformed on the nonirradiated, front region of the chloroplast before and during movement.

(D) Cp-actin filaments appeared on the chloroplast envelope in the dark-adapted cells following irradiation with strong blue light on the whole region of the chloroplast.

(E) Asymmetrically located cp-actin filaments during the avoidance response. Rapid rearrangements of the cp-actin filaments were induced by microbeam irradiation with strong blue light at the left half of chloroplast as shown in (D). Cp-actin filaments disappeared via rapid severing and random motility in the strong microbeam-irradiated region but appeared via polymerization on the leading edge of the moving chloroplast.

(F) Rearrangement of the cp-actin filaments on the chloroplast envelope during dark adaption. The cp-actin filaments reappeared around the chloroplast periphery.

(G) Legends for a cp-actin filament and light conditions. The cp-actin filament (line in blue) is shown with a cp-actin nucleator (circle in red) located on the chloroplast envelope.

[See online article for color version of this figure.]