Figure 9.

Inhibitory Effect of BDM on Blue Light–Induced Chloroplast Movement and Cp-Actin Filament Dynamics.

(A) Reorganization of the cp-actin filaments in leaves untreated (0 mM) (i to iv) or treated with 50 mM BDM for 1 h (v to viii) or 2 h (ix to xii). Time-lapse images of the avoidance response were collected at 31.2-s intervals for 15 min and 37 s under induction with 458-nm laser scans (in the 10 × 25-µm region indicated by the blue rectangle in [B]) during the intervals between the image acquisitions. The images are false-colored to indicate GFP (green) and chlorophyll (red) fluorescence. The time points at which the images were obtained are shown in the top left corner of the pictures. See also the full time-lapse series in Supplemental Movie 15 online for 0 mM (1 h), Supplemental Movie 16 online for 50 mM (1 h), and Supplemental Movie 17 online for 50 mM (2 h) BDM treatments. Bars = 25 µm.

(B) The paths of the individual chloroplast movements are shown as traces of the centers of the representative chloroplasts on the chlorophyll fluorescence images for 0 mM (i), 50 mM BDM for 1 h (ii), and 50 mM BDM for 2 h (iii). Bars = 25 µm.