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. 2013 Feb 12;25(2):637–648. doi: 10.1105/tpc.112.107896

Figure 4.

Figure 4.

CDKG1 Is Associated with the Intron 6 Region of CalS5 pre-mRNA.

(A) and (C) PCDKG1:CDKG1-Myc and PCDKG1:CDKG1-GFP constructs used for genetic complementation assays.

(B) and (D) siliques and stained anthers of cdkg1 plants bearing PCDKG1:CDKG1-Myc (B) or PCDKG1:CDKG1-GFP (D).

(E) and (F) RIP RT-PCR analysis of a PCDKG1:CDKG1-Myc line. Lanes 1, 3, and 5, the wild type; lanes 2, 4, and 6, PCDKG1:CDKG1-Myc line; lane 7, wild-type genomic DNA; lane 8, water.

(G) and (H) RIP RT-PCR analysis of a PCDKG1:CDKG1-GFP line. Lanes 1, 3, and 5, the wild type; lanes 2, 4, and 6, PCDKG1:CDKG1-GFP line; lane 7, wild-type genomic DNA; lane 8, water.

(I) RIP RT-PCR analysis of the RIP cDNA of the PCDKG1:CDKG1-Myc line using different primer pairs for CalS5. R, RIP cDNA; G, wild-type genomic DNA.