Regulation of the SPIK-Mediated K+in Currents by CPK11 and CPK24.
(A) Representative K+in currents recorded in pollen tube protoplasts of wild-type plants and the spik-1 mutant. The data points for the current-voltage curves are presented as means ± se (the wild type, n = 7; spik-1, n = 14).
(B) Whole-cell recordings of K+in currents in COS cells expressing different combinations of SPIK and CDPKs. The data points for the current-voltage curves are presented as means ± se (SPIK, n = 12; SPIK+CPK11+CPK24, n = 16; SPIK+CPK11, n = 7; SPIK+CPK24, n = 8; SPIK+CPK4+CPK10, n = 8).
(C) Whole-cell recordings of K+in currents in COS cells expressing different combinations of SPIK and the variants of CDPK. The data points for the current-voltage curves are presented as means ± se (SPIK, n = 15; SPIK+CPK11+CPK24, n = 15; SPIK+CPK11 F306A, n = 19; SPIK+CPK11+CPK24 D190A, n = 22; SPIK+CPK11 D150A+CPK24, n = 12).