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. 2013 Feb 28;25(2):649–661. doi: 10.1105/tpc.112.103184

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© 2013 American Society of Plant Biologists. All rights reserved.

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Figure 6. — Regulation of the SPIK-Mediated K⁺_in Currents by CPK11 and CPK24.

(A) Representative K⁺_in currents recorded in pollen tube protoplasts of wild-type plants and the spik-1 mutant. The data points for the current-voltage curves are presented as means ± se (the wild type, n = 7; spik-1, n = 14).

(B) Whole-cell recordings of K⁺_in currents in COS cells expressing different combinations of SPIK and CDPKs. The data points for the current-voltage curves are presented as means ± se (SPIK, n = 12; SPIK+CPK11+CPK24, n = 16; SPIK+CPK11, n = 7; SPIK+CPK24, n = 8; SPIK+CPK4+CPK10, n = 8).

(C) Whole-cell recordings of K⁺_in currents in COS cells expressing different combinations of SPIK and the variants of CDPK. The data points for the current-voltage curves are presented as means ± se (SPIK, n = 15; SPIK+CPK11+CPK24, n = 15; SPIK+CPK11 F306A, n = 19; SPIK+CPK11+CPK24 D190A, n = 22; SPIK+CPK11 D150A+CPK24, n = 12).